The Natural Asset Carbon Assessment Guide and Toolbox (NACAGT) provides standardized methods for doing carbon assessments in Central and Southern Ontario. Along with background information on carbon assessments, the document includes a database of area-based carbon sequestration rates and a list of tools for performing carbon assessments and guidance for each use. This is version 2.0 of the NACAGT with an updated database and a new method for scoring new carbon sequestration rates.